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<blockquote data-quote="Bodisky" data-source="post: 211646" data-attributes="member: 38492"><p>After dissecting hundreds of nematode-infested tarantulas, I concluded that the nematodes entered through the mouth. Once there they multiply. It was not unusual to find several stages of nematodes in one sample from the mouth. Nematodes thrive in the moist environment, once that is gone, they move to the chelicera tissue. I have never found nematodes in any other area other than the mouth and chelicerae. The symptoms of a nematode infestation in tarantulas are constant drinking and soaking the mouth. In the end-stage, they start to submerge completely in their water dish. I am guessing, at this point, the tarantula, unable to eat or drink, feels the effects of desiccation, and naturally goes to the water. I also found viable nematodes in the water dishes of an afflicted tarantula. As far as the life cycle before it enters the tarantula is unknown or was at the time. We sent a sample to the USDA. They were guessing it looked like Steinernema but complete ID/classification was not possible. I felt it was a nematode with nictitating abilities. I still have the nematode microscopic photos if you are interested. I would love your input. I discovered that nematodes illuminate under polarized light and that made them easy to find. After eight years I had to back off from the project. It was heartbreaking, every aspect of it, from helplessly watching the T die and then dissecting it. I hope someone else picked up the baton. Now I am using my polarizing microscopes to photograph molts and categorize fang structures. Fang structures vary from species to species. My group picture is a P. murinas eye structure from a molt.</p></blockquote><p></p>
[QUOTE="Bodisky, post: 211646, member: 38492"] After dissecting hundreds of nematode-infested tarantulas, I concluded that the nematodes entered through the mouth. Once there they multiply. It was not unusual to find several stages of nematodes in one sample from the mouth. Nematodes thrive in the moist environment, once that is gone, they move to the chelicera tissue. I have never found nematodes in any other area other than the mouth and chelicerae. The symptoms of a nematode infestation in tarantulas are constant drinking and soaking the mouth. In the end-stage, they start to submerge completely in their water dish. I am guessing, at this point, the tarantula, unable to eat or drink, feels the effects of desiccation, and naturally goes to the water. I also found viable nematodes in the water dishes of an afflicted tarantula. As far as the life cycle before it enters the tarantula is unknown or was at the time. We sent a sample to the USDA. They were guessing it looked like Steinernema but complete ID/classification was not possible. I felt it was a nematode with nictitating abilities. I still have the nematode microscopic photos if you are interested. I would love your input. I discovered that nematodes illuminate under polarized light and that made them easy to find. After eight years I had to back off from the project. It was heartbreaking, every aspect of it, from helplessly watching the T die and then dissecting it. I hope someone else picked up the baton. Now I am using my polarizing microscopes to photograph molts and categorize fang structures. Fang structures vary from species to species. My group picture is a P. murinas eye structure from a molt. [/QUOTE]
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