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Message: <blockquote data-quote="roj" data-source="post: 182577" data-attributes="member: 4097"><p>The best way to tell the sex is to examine closely the freshly moulted excuvia for the presence of spermatheca. This can be done with a hand lens or better still a microscope. Using a microscope can often (not always)  identify the sex of even the smallest of spiderlings. Considering the size of yours confirmation of sex via external examination would be incredibly difficult if not almost impossible. If you have  a freshly moulted excuvia, I would be more than happy to look at it under a microscope if you wanted some idea. I have attached an image of the spermatheca of a H. chilensis female (4 - 4.5 cm diagonal leg span - 5.5years old)</p></blockquote><p></p>